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Unable to Connect via Windows Based Authentication

I am having an issue connecting to our SharePoint site when using Windows Based authentication.  The following is the error I am getting:

Server Message: Server was unable to process --> Access is denied
Exception from HRESULT: 0x80070005 (E_ACCESSDENIED)

When when I create a new "site" and use forms based authentication I can log in just fine.  I can also use the Windows Based profile and log in as well.  As soon as I delete the forms based site (keeping the account associated with it) I can no longer log in using the windows based site.

Any ideas why this error might be occurring?